Sea bass ( Dicentrarchus labrax L.) vitellogenin. II—Validation of an enzyme-linked immunosorbent assay (ELISA)
Identifieur interne : 000248 ( France/Analysis ); précédent : 000247; suivant : 000249Sea bass ( Dicentrarchus labrax L.) vitellogenin. II—Validation of an enzyme-linked immunosorbent assay (ELISA)
Auteurs : E. Ma An S [Espagne] ; J. Nú Ez ; S. Zanuy [Espagne] ; M. Carrillo [Espagne] ; Françoise Le Menn [France]Source :
- Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology [ 0305-0491 ] ; 1994.
Abstract
In this study, a competitive ELISA was developed for the quantification of vitellogenin (VTG) in sea bass. The purity of the antigen (VTG) and the specificity of the antibody (AbVTG) used in the assay, were assessed by ELISA and radial immunodiffusion, and no crossreactivity was observed with the male plasma. The sensitivity range of the ELISA was 1–60 ng/ml (85-20% of binding). The intra- and inter-assay variations (at 50% of binding) were 5.3% (N = 5) and 9.8% (N = 9), respectively. Annual VTG levels were quantified in animals reared under fish farming conditions and the VTG levels were correlated with the oocyte development.
Url:
- https://api.istex.fr/document/F7DC129262A138DBDEB44D92E23EA3AC6D7357E3/fulltext/pdf
- https://api.istex.fr/document/3AD0615141510E84CAF7FDC191C0C82BFA71E40B/fulltext/pdf
DOI: 10.1016/0305-0491(94)90042-6
Affiliations:
- Espagne, France
- Aquitaine, Nouvelle-Aquitaine
- Bordeaux, Talence
- Université Bordeaux I, Université de Bordeaux
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000854
- to stream Istex, to step Curation: 000852
- to stream Istex, to step Checkpoint: 001053
- to stream Main, to step Merge: 001C19
- to stream Istex, to step Corpus: 000C07
- to stream Istex, to step Curation: 000C05
- to stream Istex, to step Checkpoint: 001052
- to stream Main, to step Merge: 001C18
- to stream Main, to step Curation: 001A96
- to stream Main, to step Exploration: 001A96
- to stream France, to step Extraction: 000248
Links to Exploration step
ISTEX:F7DC129262A138DBDEB44D92E23EA3AC6D7357E3Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Sea bass ( Dicentrarchus labrax L.) vitellogenin. II—Validation of an enzyme-linked immunosorbent assay (ELISA)</title>
<author><name sortKey="Ma An S, E" sort="Ma An S, E" uniqKey="Ma An S E" first="E." last="Ma An S">E. Ma An S</name>
</author>
<author><name sortKey="Nu Ez, J" sort="Nu Ez, J" uniqKey="Nu Ez J" first="J." last="Nú Ez">J. Nú Ez</name>
</author>
<author><name sortKey="Zanuy, S" sort="Zanuy, S" uniqKey="Zanuy S" first="S." last="Zanuy">S. Zanuy</name>
</author>
<author><name sortKey="Carrillo, M" sort="Carrillo, M" uniqKey="Carrillo M" first="M." last="Carrillo">M. Carrillo</name>
</author>
<author><name sortKey="Le Menn, F" sort="Le Menn, F" uniqKey="Le Menn F" first="F." last="Le Menn">Françoise Le Menn</name>
<affiliation><country>France</country>
<placeName><settlement type="city">Bordeaux</settlement>
<region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
</placeName>
<orgName type="university" n="3">Université Bordeaux I</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Bordeaux</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:F7DC129262A138DBDEB44D92E23EA3AC6D7357E3</idno>
<date when="1994" year="1994">1994</date>
<idno type="doi">10.1016/0305-0491(94)90042-6</idno>
<idno type="url">https://api.istex.fr/document/F7DC129262A138DBDEB44D92E23EA3AC6D7357E3/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000854</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000854</idno>
<idno type="wicri:Area/Istex/Curation">000852</idno>
<idno type="wicri:Area/Istex/Checkpoint">001053</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001053</idno>
<idno type="wicri:doubleKey">0305-0491:1994:Ma An S E:sea:bass:dicentrarchus</idno>
<idno type="wicri:Area/Main/Merge">001C19</idno>
<idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:3AD0615141510E84CAF7FDC191C0C82BFA71E40B</idno>
<idno type="url">https://api.istex.fr/document/3AD0615141510E84CAF7FDC191C0C82BFA71E40B/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000C07</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C07</idno>
<idno type="wicri:Area/Istex/Curation">000C05</idno>
<idno type="wicri:Area/Istex/Checkpoint">001052</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001052</idno>
<idno type="wicri:doubleKey">0305-0491:1994:Ma An S E:sea:bass:dicentrarchus</idno>
<idno type="wicri:Area/Main/Merge">001C18</idno>
<idno type="wicri:Area/Main/Curation">001A96</idno>
<idno type="wicri:Area/Main/Exploration">001A96</idno>
<idno type="wicri:Area/France/Extraction">000248</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Sea bass ( Dicentrarchus labrax L.) vitellogenin. II—Validation of an enzyme-linked immunosorbent assay (ELISA)</title>
<author><name sortKey="Ma An S, E" sort="Ma An S, E" uniqKey="Ma An S E" first="E." last="Ma An S">E. Ma An S</name>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón</wicri:regionArea>
<wicri:noRegion>Castellón</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nu Ez, J" sort="Nu Ez, J" uniqKey="Nu Ez J" first="J." last="Nú Ez">J. Nú Ez</name>
<affiliation><wicri:noCountry code="subField">Coast</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Zanuy, S" sort="Zanuy, S" uniqKey="Zanuy S" first="S." last="Zanuy">S. Zanuy</name>
<affiliation></affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón</wicri:regionArea>
<wicri:noRegion>Castellón</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Carrillo, M" sort="Carrillo, M" uniqKey="Carrillo M" first="M." last="Carrillo">M. Carrillo</name>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Instituto de Acuicultura de Torre de la Sal, Ribera de Cabanes, 12595 Torre de la Sal, Castellón</wicri:regionArea>
<wicri:noRegion>Castellón</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Le Menn, F" sort="Le Menn, F" uniqKey="Le Menn F" first="F." last="Le Menn">Françoise Le Menn</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biologie Marine, Université de Bordeaux I, Av. des Facultés, 33405 Talence Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Talence</settlement>
</placeName>
<placeName><settlement type="city">Bordeaux</settlement>
<region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
</placeName>
<orgName type="university" n="3">Université Bordeaux I</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Bordeaux</orgName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology</title>
<title level="j" type="abbrev">CBBOLD</title>
<idno type="ISSN">0305-0491</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1994">1994</date>
<biblScope unit="volume">107</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="217">217</biblScope>
<biblScope unit="page" to="223">223</biblScope>
</imprint>
<idno type="ISSN">0305-0491</idno>
</series>
<idno type="istex">F7DC129262A138DBDEB44D92E23EA3AC6D7357E3</idno>
<idno type="DOI">10.1016/0305-0491(94)90042-6</idno>
<idno type="PII">0305-0491(94)90042-6</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0305-0491</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In this study, a competitive ELISA was developed for the quantification of vitellogenin (VTG) in sea bass. The purity of the antigen (VTG) and the specificity of the antibody (AbVTG) used in the assay, were assessed by ELISA and radial immunodiffusion, and no crossreactivity was observed with the male plasma. The sensitivity range of the ELISA was 1–60 ng/ml (85-20% of binding). The intra- and inter-assay variations (at 50% of binding) were 5.3% (N = 5) and 9.8% (N = 9), respectively. Annual VTG levels were quantified in animals reared under fish farming conditions and the VTG levels were correlated with the oocyte development.</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
<li>France</li>
</country>
<region><li>Aquitaine</li>
<li>Nouvelle-Aquitaine</li>
</region>
<settlement><li>Bordeaux</li>
<li>Talence</li>
</settlement>
<orgName><li>Université Bordeaux I</li>
<li>Université de Bordeaux</li>
</orgName>
</list>
<tree><noCountry><name sortKey="Nu Ez, J" sort="Nu Ez, J" uniqKey="Nu Ez J" first="J." last="Nú Ez">J. Nú Ez</name>
</noCountry>
<country name="Espagne"><noRegion><name sortKey="Ma An S, E" sort="Ma An S, E" uniqKey="Ma An S E" first="E." last="Ma An S">E. Ma An S</name>
</noRegion>
<name sortKey="Carrillo, M" sort="Carrillo, M" uniqKey="Carrillo M" first="M." last="Carrillo">M. Carrillo</name>
<name sortKey="Zanuy, S" sort="Zanuy, S" uniqKey="Zanuy S" first="S." last="Zanuy">S. Zanuy</name>
</country>
<country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Le Menn, F" sort="Le Menn, F" uniqKey="Le Menn F" first="F." last="Le Menn">Françoise Le Menn</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000248 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000248 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= France |étape= Analysis |type= RBID |clé= ISTEX:F7DC129262A138DBDEB44D92E23EA3AC6D7357E3 |texte= Sea bass ( Dicentrarchus labrax L.) vitellogenin. II—Validation of an enzyme-linked immunosorbent assay (ELISA) }}
This area was generated with Dilib version V0.6.27. |